Coding

Part:BBa_K3468046

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase Y146R

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from Y to R at 146 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds,stabilized α3 helix, α3-β5loop The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 143-SER. after it was mutated to ARG, it formed hydrogen bond with 138-ASN, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase.

File:Y146R.png
Fig.1 Y146R in PyMOL
[edit]
Categories
Parameters
None